霊長類ES/iPS細胞用培地（Primate ES Cell Medium）

製品概要

≪ロット単位のまとめ買いに最適な大変お得な5本セット (25%OFF) もございます≫

京都大学iPS細胞研究所 (CiRA) の山中伸弥教授が発表された、ヒトiPS細胞樹立、培養に関する論文 (Cell 131, 1-12(2007)) でも利用された、リプロセルブランドのヒトiPS細胞、ヒトES細胞の培養に最適な培地です。

　【特徴】
・全ロットで、iPS細胞による培養試験、浸透圧、pH、滅菌、マイコプラズマ検査を行っておりますので、安心してお使いいただけます。
・血清は不含です。

注1） bFGFは含まれておりませんので、ヒトES細胞、ヒトiPS細胞培養時は添加してください。

※保存温度：-20℃以下

≪概要≫

当社霊長類ES細胞用培地は、京都大学中辻憲夫教授 (iCeMS)・末盛博文准教授 (再生医科学研究所) により開発されたヒトES細胞専用培地の組成をベースに厳密な品質管理の元で製造した製品です。全ロットにおいて、培養試験および浸透圧、pH、滅菌、マイコプラズマ検査を行っておりますので安心してお使いいただけます。

現在、これら中辻憲夫教授・末盛博文准教授の開発された培養方法は、ヒトES細胞・ヒトiPS細胞に共通のゴールドスタンダードとなっており、当社の培地も幅広く使われています。

霊長類ES/iPS細胞用培地（Primate ES Cell Medium）

ヒトiPS細胞　201B7株　霊長類ES細胞用培地 (RCHEMD001) +5 ng/mL bFGF (RCHEOT002) とフィーダー細胞 (MEF) (RCHEFC003) を用いて培養

【動画】霊長類ES/iPS細胞用培地 (RCHEMD001) 中での細胞の増殖

製品リスト

品番	製品名
RCHEMD001	Primate ES Cell Medium（500 mL）
RCHEMD001A	Primate ES Cell Medium（500 mL）x5
RCHEMD001B	Primate ES Cell Medium（500 mL）x5　＆　bFGF（25 ug） Pack

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霊長類ES/iPS細胞用培地 (Primate ES Cell Medium) 製品安全データシート

操作動画

1. ヒトiPS細胞の形態

iPS細胞の形態について解説します。細胞の状態の判断にご活用ください。良い状態のiPS細胞は、コロニーの輪郭が明瞭で内部の細胞密度が高くなっています

2. フィーダー細胞の播種方法

iPS細胞の培養に使用するフィーダー細胞は、その播種密度が重要になります。3×10⁴細胞/cm²が目安になります。

3. ヒトiPS細胞の継代方法

Dissociation Solution for human ES/iPS Cells (CTK溶液) を用いることで、高生存率かつ簡便な継代が可能になります。ピペッティング操作だけで、適度な大きさのコロニーにほぐれ、そのまま継代できます。通常3－4日に1回、継代を行います。

4. 細胞の凍結保存方法と融解方法3. ヒトiPS細胞の継代方法

Freezing Medium for human ES/iPS Cellsを使用し、ガラス化法で凍結することで、高い生存率での凍結保存が可能になります。ガラス化法とは、細胞を液体窒素で急速凍結する方法で、細胞へのダメージを最小限に抑えます。また、融解時も、温めておいた培養液を凍結細胞に直接加えることで急速解凍を行います。凍結・融解の作業に時間がかかると、生存率が低下するのでご注意ください。

文献・発表

霊長類ES/iPS細胞用培地 (Primate ES Cell Medium) を実験にご使用いただいた論文のリストはこちらです。

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