Repro Naive
製品概要

≪ロット単位のまとめ買いに最適な大変お得な5本セット (25%OFF) もございます≫

京都大学iPS細胞研究所 (CiRA) の山中伸弥教授が発表された、ヒトiPS細胞樹立、培養に関する論文 (Cell 131, 1-12(2007)) でも利用された、リプロセルブランドのヒトiPS細胞、ヒトES細胞の培養に最適な培地です。


【特徴】

 ・全ロットで、iPS細胞による培養試験、浸透圧、pH、滅菌、マイコプラズマ検査を行っておりますので、安心してお使いいただけます。
 ・血清は不含です。

注1) bFGFは含まれておりませんので、ヒトES細胞、ヒトiPS細胞培養時は添加してください。

※保存温度:-20℃以下

≪概要≫

当社霊長類ES細胞用培地は、京都大学 中辻憲夫教授 (iCeMS)・末盛博文准教授 (再生医科学研究所) により開発されたヒトES細胞専用培地の組成をベースに厳密な品質管理の元で製造した製品です。全ロットにおいて、培養試験および浸透圧、pH、滅菌、マイコプラズマ検査を行っておりますので安心してお使いいただけます。

現在、これら中辻憲夫教授・末盛博文准教授の開発された培養方法は、ヒトES細胞・ヒトiPS細胞に共通のゴールドスタンダードとなっており、当社の培地も幅広く使われています。

霊長類ES/iPS細胞用培地(Primate ES Cell Medium)

ヒトiPS細胞 201B7株 霊長類ES細胞用培地 (RCHEMD001) +5 ng/mL bFGF (RCHEOT002) とフィーダー細胞 (MEF) (RCHEFC003) を用いて培養


【動画】霊長類ES/iPS細胞用培地 (RCHEMD001) 中での細胞の増殖

製品リスト・価格

品番 製品名 希望小売価格
(税抜)
RCHEMD001 Primate ES Cell Medium(500 mL) ¥29,400
RCHEMD001A Primate ES Cell Medium(500 mL)x5  ¥110,000
(¥22,000/本)
RCHEMD001B Primate ES Cell Medium(500 mL)x5 & bFGF(25 ug) Pack
・Primate ES Cell Medium(500 mL)x5 
・bFGF(25 ug)
¥116,400
(※bFGF半額)

※RCHEMD001Bは、bFGF(25ug)を半額にしたセットです。


データシート

製品安全データシート

操作動画

1. ヒトiPS細胞の形態

iPS細胞の形態について解説します。細胞の状態の判断にご活用ください。良い状態のiPS細胞は、コロニーの輪郭が明瞭で内部の細胞密度が高くなっています。

2. フィーダー細胞の播種方法

iPS細胞の培養に使用するフィーダー細胞は、その播種密度が重要になります。3×104細胞/cm2が目安になります。

3. ヒトiPS細胞の継代方法

Dissociation Solution for human ES/iPS Cells (CTK溶液) を用いることで、高生存率かつ簡便な継代が可能になります。ピペッティング操作だけで、適度な大きさのコロニーにほぐれ、そのまま継代できます。通常3-4日に1回、継代を行います。

4. 細胞の凍結保存方法と融解方法

Freezing Medium for human ES/iPS Cellsを使用し、ガラス化法で凍結することで、高い生存率での凍結保存が可能になります。ガラス化法とは、細胞を液体窒素で急速凍結する方法で、細胞へのダメージを最小限に抑えます。また、融解時も、温めておいた培養液を凍結細胞に直接加えることで急速解凍を行います。凍結・融解の作業に時間がかかると、生存率が低下するのでご注意ください。

文献・発表

アプリケーション例

  ⇒ Efficient Embryoid Body Formation from Human iPS Cells on Novel Microfabric Vessels
  (2015年6月開催: ISSCR 2015(国際幹細胞学会))(2015年7月)


参考文献

霊長類ES/iPS細胞用培地 (Primate ES Cell Medium) を実験にご使用いただいた論文のリストはこちらです。

  • Fukusumi H, Shofuda T, Bamba Y, Yamamoto A, Kanematsu D, Handa Y, Okita K,Nakamura M, Yamanaka S, Okano H, Kanemura Y. Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins. Stem Cells Int. 2016;2016:7235757. doi: 10.1155/2016/7235757. Epub 2016 Apr 26. PubMed PMID: 27212953; PubMed Central PMCID: PMC4861799.
  • D’Souza SS, Maufort J, Kumar A, Zhang J, Smuga-Otto K, Thomson JA, Slukvin II. GSK3β Inhibition Promotes Efficient Myeloid and Lymphoid Hematopoiesis from Non-human Primate-Induced Pluripotent Stem Cells. Stem Cell Reports. 2016 Feb 9;6(2):243-56. doi: 10.1016/j.stemcr.2015.12.010. Epub 2016 Jan 21. PubMed PMID: 26805448.
  • Araoka, Toshikazu, et al. “Efficient and Rapid Induction of Human iPSCs/ESCs into Nephrogenic Intermediate Mesoderm Using Small Molecule-Based Differentiation Methods.” PloS one 9.1 (2014): e84881.
  • Diederichs, Solvig, and Rocky S. Tuan. “Functional Comparison of Human Induced Pluripotent Stem Cell-Derived Mesenchymal Cells and Bone Marrow-Derived Mesenchymal Stromal Cells from the Same Donor.” Stem cells and development ja (2014).
  • Hirata, Nao, et al. “A Chemical Probe that Labels Human Pluripotent Stem Cells.” Cell reports 6.6 (2014): 1165-1174.
  • Kanemura, Hoshimi, et al. “Tumorigenicity Studies of Induced Pluripotent Stem Cell (iPSC)-Derived Retinal Pigment Epithelium (RPE) for the Treatment of Age-Related Macular Degeneration.” PloS one 9.1 (2014): e85336.
  • Kimbrel, Erin A., et al. “Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic properties.” Stem cells and development ja (2014).
  • Koh, Sehwon, and Jorge A. Piedrahita. “From “ES-like” cells to induced pluripotent stem cells: A historical perspective in domestic animals.” Theriogenology 81.1 (2014): 103-111.
  • Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T. TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues. Sci Rep. 2016 Feb 18;6:21747. doi: 10.1038/srep21747. PubMed PMID: 26888607.
  • Morishima, Tatsuya, et al. “Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis.” Haematologica 99.1 (2014): 19-27.
  • Takahashi, Daisuke, et al. “Electroporation of Adherent Cells by Direct Lamination of Hydrogel-Based Microelectrode Substrates.” Chemistry Letters 43.4 (2014): 444-446.
  • Fukusumi, Hayato, et al. “Feeder-free generation and long-term culture of human induced pluripotent stem cells using pericellular matrix of decidua derived mesenchymal cells.” PloS one 8.1 (2013): e55226.
  • Haraguchi, Yuji, et al. “Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering.” Journal of tissue engineering and regenerative medicine (2013).
  • Hayakawa, Kazuo, et al. “Identification of target genes of synovial sarcoma-associated fusion oncoprotein using human pluripotent stem cells.” Biochemical and biophysical research communications 432.4 (2013): 713-719.
  • Hitomi, Toshiaki, et al. “Downregulation of Securin by the variant RNF213 R4810K (rs112735431, G> A) reduces angiogenic activity of induced pluripotent stem cell-derived vascular endothelial cells from moyamoya patients.” Biochemical and biophysical research communications 438.1 (2013): 13-19.
  • Hongisto, Heidi. “Fibroblast feeder cells in human pluripotent stem cell culture and retinal differentiation-progress toward clinical cell therapy.” (2013).
  • Horii, Takuro, et al. “Generation of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system.” International journal of molecular sciences 14.10 (2013): 19774-19781.
  • Iida, K., et al. “hypoxia-enhanced Derivation of ipscs from human Dental pulp cells.” Journal of dental research 92.10 (2013): 905-910.
  • Lan, Chen-Wei, et al. “Differentiation of human embryonic stem cells into functional ovarian granulosa-like cells.” The Journal of Clinical Endocrinology & Metabolism 98.9 (2013): 3713-3723.
  • Maeda, Tadao, et al. “Retinal pigmented epithelial cells obtained from human induced pluripotent stem cells possess functional visual cycle enzymes in vitro and in vivo.” Journal of Biological Chemistry 288.48 (2013): 34484-34493.
  • Matsumoto, Yoshihisa, et al. “Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation.” Orphanet journal of rare diseases 8.1 (2013): 190.
  • Nasu, Akira, et al. “Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin.” PloS one 8.1 (2013): e53771.
  • Noguchi, Michio, et al. “In vitro characterization and engraftment of adipocytes derived from human induced pluripotent stem cells and embryonic stem cells.” Stem cells and development 22.21 (2013): 2895-2905.
  • Okita, Keisuke, et al. “An Efficient Nonviral Method to Generate Integration‐Free Human‐Induced Pluripotent Stem Cells from Cord Blood and Peripheral Blood Cells.” Stem Cells 31.3 (2013): 458-466.
  • Sakuma, Tetsushi, et al. “Efficient TALEN construction and evaluation methods for human cell and animal applications.” Genes to Cells 18.4 (2013): 315-326.
  • Takase, Osamu, et al. “The Role of NF-κB Signaling in the Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells.” PloS one 8.2 (2013): e56399.
  • Tanabe, Koji, et al. “INAUGURAL ARTICLE by a Recently Elected Academy Member: Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts.” Proceedings of the National Academy of Sciences of the United States of America 110.30 (2013): 12172.
  • Tanabe, Koji, et al. “Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts.” Proceedings of the National Academy of Sciences 110.30 (2013): 12172-12179.
  • Tanaka, Akihito, et al. “Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro.” PloS one 8.4 (2013): e61540.
  • Fujikura, J., et al. “Induced pluripotent stem cells generated from diabetic patients with mitochondrial DNA A3243G mutation.” Diabetologia 55.6 (2012): 1689-1698.
  • Jung, Dongju, et al. “Incorporation of functionalized gold nanoparticles into nanofibers for enhanced attachment and differentiation of mammalian cells.” Journal of nanobiotechnology 10.1 (2012): 1-10.
  • Kajiwara, Masatoshi, et al. “Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells.” Proceedings of the National Academy of Sciences 109.31 (2012): 12538-12543.
  • Kunisada, Yuya, et al. “Small molecules induce efficient differentiation into insulin-producing cells from human induced pluripotent stem cells.” Stem cell research 8.2 (2012): 274-284.
  • Kuroda, Takuya, et al. “Highly sensitive in vitro methods for detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human iPS cells.” PloS one 7.5 (2012): e37342.
  • Matsuura, Katsuhisa, et al. “Creation of human cardiac cell sheets using pluripotent stem cells.” Biochemical and biophysical research communications 425.2 (2012): 321-327.
  • Nakahata, Tatsutoshi, et al. “Method for producing dendritic cells from pluripotent stem cells.” U.S. Patent Application 14/001,004.
  • Nakamura, Naoko, et al. “Feeder-free and serum-free production of hepatocytes, cholangiocytes, and their proliferating progenitors from human pluripotent stem cells: application to liver-specific functional and cytotoxic assays.” Cellular Reprogramming (Formerly” Cloning and Stem Cells”) 14.2 (2012): 171-185.
  • Okahara-Narita, Junko, et al. “Induction of pluripotent stem cells from fetal and adult cynomolgus monkey fibroblasts using four human transcription factors.” Primates 53.2 (2012): 205-213.
  • Shinozawa, Tadahiro, et al. “A novel purification method of murine embryonic stem cell?and human-induced pluripotent stem cell?derived cardiomyocytes by simple manual dissociation.” Journal of biomolecular screening 17.5 (2012): 683-691.
  • Shinozawa, Tadahiro, et al. “Determination of Appropriate Stage of Human-Induced Pluripotent Stem Cell?Derived Cardiomyocytes for Drug Screening and Pharmacological Evaluation In Vitro.” Journal of biomolecular screening 17.9 (2012): 1192-1203.
  • Sonoyama, Takuhiro, et al. “Differentiation of human embryonic stem cells and human induced pluripotent stem cells into steroid-producing cells.” Endocrinology 153.9 (2012): 4336-4345.
  • Tanaka, Takayuki, et al. “Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery.” Blood 120.6 (2012): 1299-1308.
  • Wada, Tamaki, et al. “Amyotrophic lateral sclerosis model derived from human embryonic stem cells overexpressing mutant superoxide dismutase 1.” Stem cells translational medicine 1.5 (2012): 396-402.
  • Wakao, Shohei, et al. “Morphologic and gene expression criteria for identifying human induced pluripotent stem cells.” PloS one 7.12 (2012): e48677.
  • Wutz, Anton. “Epigenetic alterations in human pluripotent stem cells: a tale of two cultures.” Cell stem cell 11.1 (2012): 9-15.
  • Iwabuchi, Kumiko A., et al. “ECAT11/L1td1 Is Enriched in ESCs and Rapidly Activated During iPSCGeneration, but It Is Dispensable for the Maintenance and Induction of Pluripotency.” PloS one 6.5 (2011): e20461.
  • Kobayashi, Hideyuki. “Pluripotent Stem Cells Induced from Testicular Tissue of a Man with Klinefelter Syndrome (47, XXY) by Four Transcription Factors (OCT4, SOX2, KLF4, and C-MYC).” (2011).
  • Maekawa, Momoko, et al. “Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1.” Nature 474.7350 (2011): 225-229.
  • Ogawa, Shin-ichiro, et al. “Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells.” In Vitro Cellular & Developmental Biology-Animal 47.7 (2011): 464-469.
  • Okita, Keisuke, et al. “A more efficient method to generate integration-free human iPS cells.” Nature methods 8.5 (2011): 409-412.
  • Takata, Akemi, et al. “Direct differentiation of hepatic cells from human induced pluripotent stem cells using a limited number of cytokines.” Hepatology international 5.4 (2011): 890-898.
  • Tatsumi, Rie, et al. “Simple and highly efficient method for production of endothelial cells from human embryonic stem cells.” Cell transplantation 20.9 (2011): 1423-1430.
  • Yahata, Naoki, et al. “Anti-Aβ drug screening platform using human iPS cell-derived neurons for the treatment of Alzheimer’s disease.” PLoS One 6.9 (2011): e25788.
  • Fujioka, Tsuyoshi, et al. “Establishment of induced pluripotent stem cells from human neonatal tissues.” Human cell 23.3 (2010): 113-118.
  • Kazuki, Yasuhiro, et al. “Complete genetic correction of ips cells from Duchenne muscular dystrophy.” Molecular Therapy 18.2 (2010): 386-393.
  • Lee, Tae-Hee, et al. “Functional recapitulation of smooth muscle cells via induced pluripotent stem cells from human aortic smooth muscle cells.” Circulation research 106.1 (2010): 120-128.
  • Miyoshi, Keiko, et al. “Generation of human induced pluripotent stem cells from oral mucosa.” Journal of bioscience and bioengineering 110.3 (2010): 345-350.
  • Otsuji, Tomomi G., et al. “Progressive maturation in contracting cardiomyocytes derived from human embryonic stem cells: Qualitative effects on electrophysiological responses to drugs.” Stem cell research 4.3 (2010): 201-213.
  • Sakurai, Kenji, et al. “Efficient integration of transgenes into a defined locus in human embryonic stem cells.” Nucleic acids research 38.7 (2010): e96-e96.
  • Tamaoki, N, et al. “Dental pulp cells for induced pluripotent stem cell banking.” Journal of dental research 89.8 (2010): 773-778.
  • Oka, Y., et al. “293FT cells transduced with four transcription factors (OCT4, SOX2, NANOG, and LIN28) generate aberrant ES-like cells.” J Stem cell Regenerative Med 3 (2010): 149-56.
  • Tamaoki, N., et al. “Dental pulp cells for induced pluripotent stem cell banking.”Journal of dental research 89.8 (2010): 773-778.
  • Chen, Hsin-Fu, et al. “A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals.” Human Reproduction 24.1 (2009): 71-80.
  • Hong, Hyenjong, et al. “Suppression of induced pluripotent stem cell generation by the p53?p21 pathway.” Nature 460.7259 (2009): 1132-1135.
  • Nakahara, Masako, et al. “High-efficiency production of subculturable vascular endothelial cells from feeder-free human embryonic stem cells without cell-sorting technique.” Cloning and stem cells 11.4 (2009): 509-522.
  • Ohnuki, Mari, Kazutoshi Takahashi, and Shinya Yamanaka. “Generation and characterization of human induced pluripotent stem cells.” Current protocols in stem cell biology (2009): 4A-2.
  • Wada, Tamaki, et al. “Highly efficient differentiation and enrichment of spinal motor neurons derived from human and monkey embryonic stem cells.” PloS one 4.8 (2009): e6722.
  • Fusaki, Noemi, et al. “Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome.” Proceedings of the Japan Academy. Series B, Physical and biological sciences 85.8 (2008): 348-362.
  • Takahashi, Kazutoshi, et al. “Induction of pluripotent stem cells from adult human fibroblasts by defined factors.” cell 131.5 (2007): 861-872.
  • Thiel A et al.,”Human embryonic stem cell-derived mesenchymal cells preserve kidney function and extend lifespan in NZB/W F1 mouse model of lupus nephritis.” Sci Rep. 2015 Dec2;5:17685. doi: 10.1038/srep17685.